Chloroquine Autophagy In Vivo



With autophagy including LC3B-II, ATG5 and p62. Huang, Wenyuan Yin, Kelly M. Lysosomal alterations were measured by analyses of the free and total hydrolytic activities of acid β-glycerophosphatase and β-glucuronidase in either liver homogenates or large granule fractions chloroquine can improve vemurafenib sensitivity in a resistant ex vivo primary culture and provide the fi rst demonstration in a patient harboring the V600E mutation treated with vemurafenib that the addition of chloroquine can improve clinical outcomes. Soto-Pantoja2, Pamela AG.Clarke1, M. GMX1778 exerts a cytotoxic effect by decreasing the cellular level. Inhibits autophagy in a variety of cell lines 1 . Sep 20, 2016 · Chloroquine, an autophagy inhibitor, potentiates the radiosensitivity of glioma initiating cells by inhibiting autophagy and activating apoptosis Hongxing Ye ORCID: orcid.org/0000-0001-9649-812X 1. Moreover, chloroquine derivatives, such as hydroxychloroquine (HCQ), in combination with antineoplastic chemotherapeutic drugs or radiotherapy treatments inhibit multiple cancer cell types (32, 33) Finally, the in vivo combination effect of chloroquine (autophagy inhibitor) and erlotinib in EGFR-mutated lung ADC was studied in a tumor xenograft model EAD1 is a viable lead compound for evaluation of the antitumor activity of autophagy inhibitors in vivo. Aug 23, 2017 · In addition, chloroquine inhibited autophagy, and induced autophagosome and autolysosome accumulation in the cytoplasm, thus interfering with degradation; however, OSCC apoptosis was barely affected by chloroquine. Tools to manipulate and measure autophagy flux in brain in vivo are not well established. A large number of investigations indicated that excessive metal exposure can evoke apoptosis and autophagy both in vivo and in vitro. Objective: To examine the in vivo pharmacokinetics and pharmacodynamics of the lysosomal inhibitor chloroquine (CQ) and the levels of selected autophagy markers to determine usefulness of CQ as a tool to study autophagy flux in brain Here, we describe the use of monodansylcadaverine (MDC) and the lysosomotropic drug chloroquine to measure autophagic flux in in vivo model systems, specifically focusing on its use in the myocardium Sep 18, 2014 · Autophagic flux is a measure of efficiency or throughput of the pathway. Cited by: 28 Publish Year: 2015 Author: Angèle Nalbandian, Katrina J. and the lysosomotropic drug chloroquine could be used to measure autophagic flux in both in vitro and in vivo model systems In vitro and in vivo effects of MK2206 and chloroquine combination therapy on endometriosis: autophagy may be required for regrowth of endometriosis. Lys05 produced more potent antitumor activity as a single agent both in vitro and in vivo in multiple human cancer cell lines and xenograft models compared with HCQ Recently, macroautophagy/autophagy has emerged as a promising target in various types of solid tumor treatment. Nov 15, 2015 · Indeed, Chloroquine is a potent blocker of autophagy and has been demonstrated in a lab setting to dramatically enhance tumor response to radiotherapy, chemotherapy and even anti-hormonal therapy (please see references below) Chloroquine phosphate (50-63-5) is an antimalarial drug. Therefore, we conclude that activation of the P2RY12 receptor decreases cholesterol efflux and promotes VSMC-derived foam cell formation by blocking autophagy in …. The acute effects of chloroquine on rat hepatic lysosomes were evaluated both in vivo and in vitro with the isolated perfused rat liver, liver slices, homogenates and large granule fractions. In vivo effects of MK2206 + chloroquine in a mouse model of endometriosis During the experimental period, all mice survived, and no significant differences in body weight were observed among the four experimental groups Nov 14, 2013 · Accurate methods to measure autophagic activity in vivo in neurons are not available, and most chloroquine autophagy in vivo of the studies are based on correlative and static measurements of autophagy markers, leading to. HepG2 cells were stained CYTO-ID® Green Detection Reagent 2 after being cultured for 20h in DMSO (control), 0.5 µM Rapamycin (Rap), 10µM Chloroquine (CLQ), or both 0.5µM Rap and 10µM CLQ. To obtain the chloroquine autophagy in vivo antiviral effect, it is necessary to increase the chloroquine dosage An important mode of action of chloroquine and hydroxychloroquine is the interference of lysosomal activity and autophagy. Soto-Pantoja2, Pamela AG. McMasters, Lacey R. Furthermore, autophagy-specific enhanced oxaliplatin lethality of HCC in vivo and in vitro. Inhibiting autophagy …. ATG9A was consistently induced by hypoxia, and silencing of ATG9A led to decreased proliferation in vitro and delayed tumour growth in vivo leukemic effect of chloroquine. The results of the in vivo study demonstrated that chloroquine effectively inhibited OSCC tumor growth in the CAL27 xenograft model Autophagy is 1 of the physiologic processes affected by chloroquine. Yazdi, Virginia E. The mTORC-1 inhibitor and autophagy activator rapamycin enhances autophagy in cartilage and prevents tissue destruction both in vitro (16) and in murine experimental OA (17). Having demonstrated the effect of chloroquine administration in vivo, we next sought to establish whether chloroquine inhibits autophagy pathways in isolated rat PASMCs Tools to manipulate and measure autophagy flux in brain in vivo are not well established. In addition, chloroquine inhibited autophagy, and induced autophagosome and autolysosome accumulation in the cytoplasm, thus interfering with degradation; however, OSCC apoptosis was barely affected by chloroquine.