Chloroquine atm kastan


Accordingly, chloroquine impaired spontaneous lymphoma development in Atm-deficient mice, a mouse model of ataxia telangiectasia, but not in p53-deficient mice Purpose: We have previously shown that the antimalarial agent chloroquine can abrogate the lethal cellular effects of low-dose-rate (LDR) radiation in vitro, most likely by activating the ataxia-telangiectasia mutated (ATM) protein. Kastan, Kirsteen H. T0194 CAS 50-63-5 Synonyms: Aralen phosphate, Chingamin phosphate Chloroquine is an aminoquinoline antimalarial and also is widely used chloroquine atm kastan as an autophagy inhibitor. Multiple mechanisms might explain the …. 500 μM CQ (CQ) was added to both KU-and DMSO-incubated cells Dec 10, 2007 · ATMIN colocalizes with autophosphorylated ATM after treatment of cells with chloroquine or in hypotonic conditions, but ATMIN dissociates from ATM after exposure to IR. Chloroquine also improved metabolic abnormalities in ob/ob and db/db mice. In human cells, following DNA damage, ATM undergoes autophosphorylation at residue S1981 in its FAT domain, which results in the simultaneous dissociation of the ATM homodimers (Bakkenist and Kastan, 2003) (see poster). Chloroquine diphosphate Catalog No. Loehberg, Tiia Thompson, Michael B. Hydroxychloroquine and chloroquine are antimalarial drugs commonly used for the treatment of rheumatic diseases. Kastan is a Pediatric Hematologist-Oncologist in Durham, NC. He and his colleagues have subsequently discovered that low-dose, intermittent chloroquine treatment could significantly reduce tumor development in mouse models of Burkitt. Chloroquine also is an inhibitor of toll-like receptors (TLRs) and Kastan, 2003); like other PI3Ks, it requires a stimulus for activation. Liang Burns, Nathaniel Michael B Kastan's research works | Duke University, North https://www.researchgate.net/scientific-contributions/39653610_Michael_B_Kastan Michael B Kastan's 226 research works with 41,804 citations and 4,457 reads, including: Low dose chloroquine decreases insulin resistance in human metabolic syndrome but …. Nov 16, 2006 · When Kastan’s chloroquine atm kastan lab discovered that chloroquine can activate ATM, it provided a potential treatment intervention. et al. Chloroquine, an anti-malaria drug and a stimulator of ATM, is a new activator of Akt. Roles of ATM and NBS1 in chromatin structure modulation and DNA double-strand break repair hypotonic swelling or chloroquine E., Monnat, R. To determine if the latter effect is p53-dependent, we quantified atherosclerosis in whole mount aortas as well. Recent data from the Kastan lab has demonstrated that inhibition of autophagy by chloroquine blocks recycling of energy and MYC-mediated lymphomagenesis ATM activator chloroquine induces phosphorylation of KAP1 at S824 and EBV lytic replication. Ataxia Telangiectasia-Mutated and p53 Are Potential Mediators of Chloroquine-Induced Resistance to Mammary Carcinogenesis Michael B Kastan. Bakkenist, Michael B. Cited by: 7 Publish Year: 2018 Author: Joe Pelt, Joe Pelt, Sara Busatto, Sara Busatto, Mauro Ferrari, Mauro Ferrari, E. Because ATM. Banking on ATM as a new target in metabolic syndrome the authors show that chloroquine, an antimalarial drug, also activates ATM, which inhibits JNK, and improves insulin sensitivity and cardiovascular effects. Targeting chloroquine atm kastan cytoplasmic ATM for the treatment of diabetes, cancer and neuronal degeneration Type 2 and type 1 diabetes. ATM null mice from the same background were used to determine the influence of ATM. Edwards, Franc Chloroquine activates the p53 pathway and induces https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2940600 Jan 27, 2010 · Chloroquine activates the p53 pathway and induces apoptosis in human glioma cells.

Is Hydroxychloroquine Safe In Pregnancy

Much of our understanding of the mechanisms involved in cellular DNA damage response pathways has come from studies of human cancer susceptibility syndromes that are altered in DNA damage responses US5314894A US07/945,032 US94503292A US5314894A US 5314894 A US5314894 A US 5314894A US 94503292 A US94503292 A US 94503292A US 5314894 A US5314894 A US 5314894A Authority US United States Prior art keywords ethylamino amino ethanol quinolinyl pentyl Prior art date 1992-09-15 Legal status (The legal status is an assumption and is not a …. To assess whether CQ’s effects were dependent on ATM, a C2C12 cell line was used that expressed shRNA against ATM chloroquine atm kastan (expression knocked down, KD). In this study, 708 women with …. Methods and Materials: C57BL/6 mice were irradiated with a total of 12.8 Gy delivered at 9.4 cGy/hour. Cited by: 6 Publish Year: 2016 Author: Larry D. Chloroquine also is an inhibitor of toll-like receptors (TLRs).. Author summary Viruses that persist for the life of the host, like the herpesvirus Epstein-Barr virus (EBV), tightly regulate lytic replication to reduce killing of host cells and ensure virus survival. Finck,1 Jie Ren,1 Kara N. ” DNA damage activates ATM through intermolecular autophosphorylation and dimer dissociation. Maclean, Dean G. Seminal studies 2 years ago by Bak-kenist and Kastan (3) revealed that, in undamaged cells, ATM resides as a catalyt-. Jun 20, 2007 · ATM is strongly activated by DSBs, but changes in chromatin structure induced by chloroquine or hypotonic salt have been shown to also stimulate ATM activation in the absence of DSBs (Bakkenist and Kastan, 2003) Significant progress has been made in recent years in elucidating the molecular controls of cellular responses to DNA damage in mammalian cells. Serine 1981 was the first identified site of ATM autophosphorylation and antibodies specific for phosphorylated serine 1981 have been widely used as a biological marker for the active monomeric form of ATM (Bakkenist and Kastan, 2003) Figure 6 Activation of ATM by chromatin-active agents that do not cause DNA breaks. Kastan Chloroquine and nanoparticle drug delivery: A promising https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6677248 Potential mechanisms for this chloroquine-induced drug sensitization are thought to include DNA intercalation and activation of ataxia telangiectasia mutated (ATM) and p53 (Bakkenist & Kastan, 2003; Maycotte, et al., 2012; Wenzel, et al., 2010). Recent data from the Kastan lab has demonstrated that inhibition of autophagy by chloroquine blocks recycling of energy and MYC-mediated lymphomagenesis Chloroquine also improved metabolic abnormalities in ob/ob and db/db mice. We asked if chloroquine could cause phosphorylation of KAP1 and activate EBV replication Loehberg CR, Thompson T, Kastan MB, Maclean KH, Edwards DG, Kittrell FS, et al. C2C12 myotubes were placed in medium containing either 10 μM KU or chloroquine atm kastan vehicle (DMSO) for 30 min. 500 μM CQ (CQ) was added to both KU-and DMSO-incubated cells ATM activator chloroquine induces phosphorylation of KAP1 at S824 and EBV lytic replication. The Women's Environment, Cancer, and Radiation Epidemiology Study was designed to identify genetic and environmental determinants of contralateral breast cancer (CBC). ATM chloroquine ATM P P ATM P oxidative stress. …. Kastan MB. ATM null mice from the same background were used. C2C12 myotubes were placed in medium containing either 10 μM KU or vehicle (DMSO) for 30 min. Chloroquine-induced cell death in primary MEFs and human colorectal cancer cells was dependent upon p53, but not upon the p53 modulators Atm or Arf. We show that repression of EBV replication is disrupted by the antimalarial drug chloroquine which modifies an otherwise normal cellular mechanism that repairs DNA, to …. A, down-regulation of ATM abolishes chloroquine-induced p53 phosphorylation at serine 15 (24 h). To translate this observation to humans, we conducted two clinical trials of chloroquine in people with …. Jan 02, 2008 · Chloroquine-induced cell death in primary MEFs and human colorectal cancer cells was dependent upon p53, but not upon the p53 modulators Atm or Arf. Subsequently, serines 367 and. Maclean,3 Carlos Bernal-Mizrachi,1 Anthony J. Chloroquine was administered by two intraperitoneal injections of 59.4 μg per 17 g of chloroquine atm kastan body weight, 24 hours and 4 hours before irradiation. In this study, 708 women with …. William and Jane Shingleton Distinguished Professor of Pharmacology and chloroquine atm kastan Cancer Biology, Pharmacology & Cancer Biology, Basic Science Departments 2012; Professor. Chloroquine is a 4-aminoquinoline drug used in the treatment or prevention of malaria and as antinflammatory drug Chloroquine at neutral pH is not protonated and enters the cell by simple diffusion, and diffuses to acid vacuoles too.