Chloroquine Induced Autophagy


Identified the antimalarial drug chloroquine (CQ) as a potent inducer of Par-4 secretion from normal cells under conditions that showed no normal cell death. Chloroquine-induced itching is very common among black Africans (70%), but much less common in other races. It is widely accepted that chloroquine and hydroxychloroquine accumulate in lysosomes (lysosomotropism) and inhibit their function Cowley et al. Moreover, Chloroquine inhibits autophagy as it raises the lysosomal pH, which leads to inhibition of both fusion of autophagosome with lysosome and lysosomal protein degradation [4] The induction of autophagy plays a vital catalytic role in MeHg-reduced viability and induced apoptosis in astrocytic (Yuntao et al., 2016). New therapies are urgently needed for patients with small cell lung cancer (SCLC). Both cancer cells and kidney proximal tubular cells tolerate cisplatin through the use of autophagy because autophagy protects cells, not only from DNA damage, but also from the mitochondrial reactive oxygen species induced by the … Cited by: 401 Publish Year: 2013 Author: Tomonori Kimura, Yoshitsugu Takabatake, Atsushi Takahashi, Yoshitaka Isaka Tropical Journal of Pharmaceutical Research https://www.tjpr.org/home/abstract.php?id=2832&aTitle=Autophagy inhibition by Autophagy inhibition by chloroquine prevents increase in blood pressure and preserves endothelial functions Moon Jain 1,2 , Hina Iqbal 3 , Pankaj Yadav 3 , Himalaya Singh 1,2 , Debabrata Chanda 3 , Kumaravelu Jagavelu 1,2 , Kashif Hanif 1,2. In order to verify this view, we used chloroquine to inhibit autophagy pathway. We explored the combination of ABT-737 and chloroquine, an inhibitor of autophagy…. The combination of chloroquine with zinc enhanced chloroquine's cytotoxicity and induced apoptosis in A2780 cells. To further investigate the interplay between oxidative stress and autophagy in PAMAM dendrimers-induced neuronal cell death, the effects of PAMAM dendrimers combined with autophagy inhibitors (LY294002 and chloroquine) or autophagy activators (trehalose and rapamycin) were also examined in PC-12 cells The autophagy pathway has recently been implicated in several neurodegenerative diseases. 20,21 We next investigated how the lysosomal-related effects of CQ may contribute to its ability to potentiate the anticancer activity of SAHA. Here we show that in human gallbladder carcinoma (GBC) cells lines, SGC-996 and GBC-SD, autophagy is induced by the DNA damaging agent 5-fluorouracil (5-FU). Now, Wan et al . It is increased during malaria fever; its severity is correlated to the malaria parasite load in …. While in combination with the pre-treatment of chloroquine (CQ), a inhibitor chloroquine induced autophagy of autophagy, the inhibition of 5-FU to the proliferation and viability of GBC cells was potentiated Chloroquine and other inhibitors of autophagy have cytotoxic effects on diverse leukemia‐initiating cell types such as CD34‐positive and glucocorticoid‐resistant clones. However, its effect on ischemic endothelial cells remains unclear. The suppression of oxidative phosphorylation that occurs in hypoxia causes an increase in AMP/ATP ratio and subsequent activation of AMPK. Lysosomes contain a number of proteases that participate in the autophagic. Rather, BafA1 appears to inhibit events downstream of chloroquine-induced autophagosome accumulation, such as the loss of mitochondrial or lysosomal integrity. It increases with age, and is so severe as to stop compliance with drug therapy. Further study revealed that autophagy activated by rapamycin or inhibited by chloroquine influenced the expression and nuclear translocation of Nrf2, thereby. Conclusion Together, these results suggest that Luteolin has the capacity to induce selectively apoptotic cell death both in primary cutaneous SCC cells and in metastatic SCC cells in combination with chloroquine, an inhibitor of autophagosomal degradation. Treatment of cells with the macrolide antibiotic bafilomycin A1, an inhibitor of vacuolar (V)-ATPase, or with the lysosomotropic agent chloroquine, has been shown to pharmacologically inhibit autophagy as evidenced by an accumulation of autophagosomes, which in turn causes Bax-dependent apoptosis Hydroxychloroquine and chloroquine are antimalarial drugs commonly used for the treatment chloroquine induced autophagy of rheumatic diseases. Chloroquine is a potent pulmonary. pp.

Chloroquine And Proguanil Dose


The induction of autophagy plays a vital catalytic role in MeHg-reduced viability and induced apoptosis in astrocytic (Yuntao et al., 2016). Jul 11, 2018 · Chloroquine (CQ), which is frequently used clinically as an antimalarial agent, is a classic inhibitor of autophagy that blocks the binding of autophagosomes to lysosomes by altering the acidic environment of lysosomes, resulting in the accumulation of a large number of degraded proteins in cells . Chloroquine-induced itching is very common among black Africans (70%), but much less common in other races. Antioxidants N-acetyl cysteine (NAC), lipoic acid (LA), and tocopherol were purchased from Beyotime Institute of Biotechnology (Haimen, China). In: Cancer Letters . The induction of autophagy plays a vital catalytic role in MeHg-reduced viability and induced apoptosis in astrocytic (Yuntao et al., 2016). IL-37 was preferentially induced in autophagic cells associated with LC3 conversion.. Chloroquine-induced itching is very common among black Africans (70%), but much less common in other races. Autophagy Inhibitors - Autophagy Sigma-Aldrich Inducing autophagy as an alternate way to enhance the clearance of the HD protein is a very promising strategy for preventing or delaying the onset of Huntington's Disease. role of chloroquine, an autophagy inhibitor, as a novel antitumor drug. Aug 10, 2017 · To further confirm a role of hypoxia in the outcome of anti-autophagy treatment, we assessed the efficacy of two autophagy inhibitors, chloroquine and …. Mar 19, 2020 · Very basically, chloroquine interferes with a process called autophagy (from the Greek “to eat oneself”). Bing Zhu 1*, Shanxin Li 1*, Lei Yu 3*, Wei Hu 4, Dandan Sheng 2, Jing Hou 5, Naping Zhao 1, Xiaojuan Hou 2, Yechen Wu 2, chloroquine induced autophagy Zhipeng Han 2, Lixin Wei 2 , Li Zhang 4,1 . Cited by: 2 Publish Year: 2018 Author: Baoxing Jia, Yanan Xue, Xiaoyu Yan, Jiuling Li, Yao Wu, Rui Guo, Juanjuan Zhang, Lichao Zhang, Yapin Chloroquine-induced autophagic vacuole accumulation and https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2940627 Jan 27, 2010 · Quinacrine, an acridine derivative and a well-known antimalarial drug has been proposed to be effective in killing cancer cells in part due to its ability to induce transcriptional activation of p53. 210 One potential explanation comes from the observation that CQ may …. Interestingly, inhibition of autophagy with chloroquine or 3-methyladenine obviously decreased MeHg-induced astrocytes apoptosis ratio …. Chloroquine is commonly used to study the role of endosomal acidification in cellular processes [2, 3], such as the signaling of intracellular TLRs. An important mode of action of chloroquine and hydroxychloroquine is the interference of lysosomal activity and autophagy. Autophagy can promote survival of cancer cells under stress and comprise a pathway of escape from cytotoxic therapies. Liver fibrosis in rats was induced by carbon tetrachloride (CCl 4 ). Using a mouse model of cerulein-induced pancreatitis, we found that loss of autophagy …. The autophagy level, as measured by LC3I/LC3II and autophagy flux, was increased in aortas of KL(+/−) mice The inhibition of autophagy by chloroquine or by small interfering RNA promoted bevacizumab-induced apoptosis and proliferation inhibition. Bafilomycin A1 and Rapamycin were obtained from Sangon Biotech (Shanghai, China) Chloroquine-induced itching is very common chloroquine induced autophagy among black Africans (70%), but much less common in other races. Interestingly, inhibition of autophagy with chloroquine or 3-methyladenine obviously decreased MeHg-induced astrocytes apoptosis ratio (Yuntao et al., 2016) Autophagy promotes tumor progression downstream of oncogenic KRAS, yet also restrains inflammation and dysplasia through mechanisms that remain incompletely characterized. Moreover, Chloroquine inhibits autophagy as it raises the lysosomal pH, which leads to inhibition of both fusion of autophagosome with lysosome and lysosomal protein degradation [4] The lysosomal inhibitor chloroquine (CQ) and autophagy inducer trehalose were obtained from Sigma (St Louis, MO). Hexokinase II inhibitor, 3-BrPA induced autophagy by stimulating ROS formation in human breast cancer cells Qianwen Zhang 1, Yuanyuan Zhang 1, Pei Zhang , Zhenhua Chao , Fei Xia , Chenchen Jiang 2, Xudong Zhang , Zhiwen Jiang 1, Hao Liu 1 Faculty of pharmacy, Bengbu Medical College, Bengbu, Anhui, P. Chloroquine (CQ) is a lysosomotropic agent with an extensive range of biological effects (1). Antioxidants N-acetyl cysteine (NAC), lipoic acid (LA), and tocopherol were purchased from Beyotime Institute of Biotechnology (Haimen, China). Treatment with Δ9-Tetrahydrocannabinol (THC) resulted in the activation of autophagy, loss of cell viability, and activation of apoptosis, whereas cotreatment with chloroquine or knockdown of Atg7, but not Beclin-1 or Ambra1, prevented THC-induced autophagy and cell death in vitro Trehalose administration to mice overexpressing GFP-tagged LC3 significantly increased the number of GFP-LC3 dots, both in the presence and absence of chloroquine administration. Recently, it was reported that chorein-depleted cells showed accumulation of ….

Chloroquine induced autophagy

Autophagy has been implicated in the pathogenesis of cancer cell invasion Nov 20, 2009 · An Autophagy Inhibitor, Chloroquine, Overcomes An Imatinib-Resistant T315I Mutant of Bcr/Abl, Whose Latent May Exist Before Imatinib Treatment appear to be independent of the chloroquine-induced accumulation of autophagosomes. Background: Everolimus (RAD001), an mTORC1 inhibitor, demonstrated promising, but limited, anti-cancer effect in NETs.. Bafilomycin A1 and Rapamycin were obtained from Sangon Biotech (Shanghai, China) The induction of autophagy plays a vital catalytic role in MeHg-reduced viability and induced apoptosis in astrocytic (Yuntao et al., 2016). Reprogramming the immune response toward an chloroquine induced autophagy anti-inflammatory phenotype could help limit the inflammation during liver injury. 205, 209 However, in leukemia patients, high doses of CQ are needed to inhibit autophagy, which limits their therapeutic efficacy. Research studies demon- strate that chloroquine accumulates in acidic lysosomes and increases the lysosomal pH Abrogation of Autophagy by Chloroquine Alone or in Combination with mTOR Inhibitors Induces Apoptosis in Neuroendocrine Tumor Cells. Recent studies indicate that receptor for activated C kinase 1 (RACK1) is involved in autophagosome formation in Drosophila and mice, and that it plays an essential role in morphine‐associated memory. Intrathecal injection of an autophagy inhibitor (chloroquine) reversed the analgesic effect of koumine, as well as the inhibitory effect of koumine on astrocyte activation in the spinal cord. Interestingly, inhibition of autophagy with chloroquine or 3-methyladenine obviously decreased MeHg-induced astrocytes apoptosis ratio …. Chloroquine (CQ) is an anti-malarial drug, and is also known as an inhibitor of autophagy. 249-258 Trehalose administration to mice overexpressing GFP-tagged LC3 significantly increased the number of GFP-LC3 dots, both in the presence and absence of chloroquine administration. It increases with age, and is so severe as to stop compliance with drug therapy. Bafilomycin A1 and Rapamycin were obtained from Sangon Biotech (Shanghai, China) Pathological and physiological stimuli, including acute exercise, activate autophagy; however, it is unknown whether exercise training alters basal levels of autophagy and whether autophagy …. This may contribute to novel EGFR‑targeted therapeutic strategies in the near future Aug 22, 2018 · Decreased autophagy was also observed in osteocytes of superoxide dismutase 1‐ (SOD1‐) deficient mice. Cited by: 70 Publish Year: 2012 Author: Lien Verschooten, Kathleen Barrette, Sofie Van Kelst, Noemí Rubio Romero, Charlotte Proby, Rita De V Chloroquine in Cancer Therapy: A Double-Edged Sword of https://cancerres.aacrjournals.org/content/73/1/3 Jan 01, 2013 · Thus, chloroquine, which inhibits autophagy, may promote chemotherapy-induced kidney injury through multiple pathways . Apr 15, 2020 · Chronic liver injury is characterized by sustained inflammation that can lead to organ failure. However, the mechanism underlying this ocular cytotoxicity remains to be elucidated. This study suggested that autophagy inhibition may have a role not only in combination therapies for patients with active metastatic disease, but also in the prevention of early disease Oct 09, 2019 · Manipulating pro‑survival autophagy by the combined application of chloroquine with gefitinib is a promising approach for improving the efficacy of EGFR inhibitors in cancer treatment. Reprogramming the immune response toward an anti-inflammatory phenotype could help limit the inflammation during liver injury. Medicine, The University of Arizona, 1501 N Campbell Avenue, Tucson, AZ 85724, USA In contrast, silencing of Atg3 or Atg7 or pretreatment with chloroquine increases resistance of HCC cells to starvation-induced autophagy. Oncology Letters, chloroquine induced autophagy 15, 2839-2846. For quantification, the band intensity ratio of LC3B-II/LC3B-I in each group was normalized to that in the untreated control group ( n = 3) p38β MAPK mediates ULK1-dependent induction of autophagy in skeletal muscle of tumor-bearing mice Zhelong Liu 1, 2,† , Ka Wai Thomas Sin 1,† , Hui Ding 1,3 , HoangAnh Amy Doan 1 , Song Gao 1 , Hongyu Miao 4 , Yahui Wei 1,# , Yiman Wang 1,# , Guohua Zhang 1 , and Yi-Ping Li 1. In this study, we found that there were statistically significant dose- and time-dependent increases in the number of …. It was well known that chloroquine inhibited lysosome fusion with autophagosomes and elevated lysosomal PH, thereby preventing the final digestion step and inhibiting lysosomal activity [ 21 ] Chloroquine-induced autophagic vacuole accumulation and cell death in glioma cells is p53 independent Ying Geng Departments of Pathology (Y.G., L.K., B.J.K., K.A.R.) and Cell Biology (L.K.), University of Alabama at Birmingham, Birmingham, Alabama. It is increased during malaria fever; its severity is correlated to …. Furthermore, cells pretreated with YC-1, a HIF-1α inhibitor, displayed significantly attenuated bevacizumab-induced autophagy Furthermore, rapamycin‐induced autophagy helped CESCs maintain the chondrogenic properties and inhibited extracellular matrix protease expression and osteogenic differentiation. The combined therapy of VNP20009 and chloroquine not only enhanced the bacterial tumor targeting ability but also facilitated the infiltration of immune cells into the tumor A combination of chloroquine and increasing concentrations of TQ resulted in more LC3-II compared to TQ alone, which may be due to either autophagy induction or autophagy inhibition.